Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices SpectraCuvette Adapter, 18 mm, SpectraMax compatible
The Molecular Devices SpectraCuvette Adapter (part number 5301193) adapts standard cuvettes for measurement in compatible microplate readers by positioning cuvettes at a defined plate height for accurate optical alignment and absorbance readings.
- Provides 18 mm plate height for proper optical alignment.
- Holds cuvettes securely for absorbance measurements.
- Designed to maintain plate height below instrument maximums to prevent damage.
- Uses plate-height marking to ensure correct adapter selection.
Perkin Elmer US LLC Split/Splitless Injector Starter Kit for Manual Gas Control
Includes the following pieces: (5) Ultra Inert Splitless Liners with Wool 4 mm I.D. (09200696) (5) Ultra Deactivated Split/Splitless Liners with Glass Wool, 4 mm I.D. (09200624) (10) Silicone O-Rings (09200714) (50) Green Injection Port Septa (N6621028) (10) 0.4 mm (09200685), (10) 0.5 mm (09200686) and (10) 0.8 mm Graphite/Vespel Ferrules (09200687) (2) Gold Seal Washers (N6551043) (1) Gold Seal Nut (N6552080) (1) Capillary Injector Nut (N6552084) (1) Ceramic Column Cutter (N9301376)
Medchemexpress LLC Tofacitinib 10 mM * 1 mL in DMSO, Solid + Solvent, Ready for Reconstitution
Tofacitinib is an orally available JAK3/2/1 inhibitor with IC50s of 1, 20, and 112 nM, respectively.
Molecular Devices Injector System, SpectraMax iD3 Compatible, Two Injectors
The SpectraMax iD3 Injector System adds automated reagent injection capability to iD3-series multi-mode microplate readers, enabling precise timed reagent delivery for kinetic and flash-luminescence assays.
- Adds two precision injectors for automated reagent delivery.
Molecular Devices Validation Plate, Absorbance, NIST-Traceable, ISO 17025 Recertifiable
The SpectraTest ABS2 Validation Plate from Molecular Devices is an automated absorbance validation tool used to verify the optical performance and sensitivity of absorbance-capable microplate readers. It provides NIST-traceable photometric checks and supports recertification to maintain GMP and GLP compliance; requires SoftMax Pro software version 6.5.1 or higher.
- Validates absorbance sensitivity and photometric accuracy.
- Automated certificate value entry for streamlined validation workflows.
- Compatible with absorbance-capable microplate readers and automated handling systems.
- Supports annual recertification with ISO 17025 traceability.
- Designed for GMP and GLP instrument performance verification.
- Factory recertification and repair services reduce downtime.
Revvity Health Sciences Inc HTRF Human Total H2AX Detection Kit, 500 Assay Points
HTRF Human Total H2AX Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF dsRNA (IVT) Detection Kit, 500 Assay Points
HTRF dsRNA (IVT) Detection Kit, 500 Assay Points
Molecular Devices Microplate Reader, Multi-Mode, Monochromator, Temperature Control
The SpectraMax M5e multi-mode microplate reader provides flexible, high-performance detection for absorbance, fluorescence, luminescence, time-resolved fluorescence, fluorescence polarization, and HTRF assays across 6-384 well plates and cuvettes. It features a tunable monochromator with 1 nm spectral resolution, temperature control, and adaptive detector optimization for broad dynamic range.
- Supports absorbance, fluorescence, luminescence, time-resolved fluorescence, fluorescence polarization, and HTRF assays.
- Monochromator with 1 nm wavelength selection for spectral scanning and up to six wavelengths per read.
- Temperature control from ambient +2°C to 60°C for incubated assays.
- Automated detector optimization and absorbance normalization for extended dynamic range and improved accuracy.
- Compatible with GxP-capable data acquisition and validation workflows for regulated environments.
